Phage display antibody repertoire7/24/2023 The International Journal of Tuberculosis and Lung Disease, 6, 1023–1028. Humoral immune response against 38-kDa and 16-kDa mycobacterial antigens in bone and joint tuberculosis. Brazilian Journal of Medical and Biological Research, 38, 587–596.ĭemkow, U., Zielonka, T., Nowak-Misiak, M., Filewska, M., Bialas, B., Strzalkowski, J., & Skopinska-Rozewska, E. Humoral response to low molecular weight antigens of Mycobacterium tuberculosis by tuberculosis patients and contacts. Human anti-murine immunoglobulin responses in patients receiving monoclonal antibody therapy. Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library. Rahumatullah, A., Ahmad, A., Noordin, R., & Lim, T. European Journal of Immunology, 35, 2131–2145. The human antibody repertoire specific for rabies virus glycoprotein as selected from immune libraries. Principles and application of antibody libraries for infectious diseases. The WHO 2014 global tuberculosis report-further to go. N., Baroness Masham of Ilton, Oxley, A., & Oliver, M. Zumla, A., George, A., Sharma, V., Herbert, R. Tuberculosis: A problem with persistence. American Journal of Infectious Diseases and Microbiology, 3, 18–31. Tuberculosis is a fatal disease among some developing countries of the world. The developed library can be applied against other TB antigens and aid antibody-derived TB immunity studies in the future. This strengthens the role of antibodies in TB immunity in addition to the role played by cellular immunity. Biopanning of the library isolated three monoclonal antibodies with unique gene usage. The immune library with an estimated diversity of 10 9 independent clones was then applied for the identification of monoclonal antibodies against Mycobacterium tuberculosis α-crystalline as a model antigen. This study describes the generation of an immune single-chain variable fragment (scFv) library derived from TB-infected individuals. The development of disease-specific antibody libraries or immune libraries is useful to better understand antibody-mediated immune responses against specific disease antigens. Phage display is an intriguing approach that can be utilized to study antibody-mediated responses against a particular infection via harvesting the B cell repertoire from infected individuals. Therefore, the ability to correlate the natural antibody responses of infected individuals toward TB antigens would help strengthen this concept. However, recent studies has helped position humoral immunity especially antibodies back into the foray in relation to TB immunity. B cells and in particular antibodies has always played second fiddle to cellular immunity in regard to tuberculosis (TB).
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